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为研究毕节烟区不同产地烟叶感官质量的差异，并明确有关键影响的香气成分，以毕节烟区8个县（区、市）的42份C3F烤烟作为材料，采用感官质量评价和HS-SPME-GC-MS技术对毕节烟叶的感官质量和香气成分进行分析。结果表明：(1)毕节不同产地烟叶在感官品质方面存在较明显的差异，中、西部产地烟叶质量整体优于东部产地。(2)共定性到318种挥发性成分，结合OPLS-DA模型分析和rOAV分析，筛选出13种VIP>1且rOAV>1的香气成分，进一步通过与感官质量指标的相关性分析，确认β-大马酮、香叶基丙酮、戊酸叶醇酯、2,5-二甲基-4-羟基-3(2H)-呋喃酮、2-苯乙醇和苯乙醛是使毕节不同产地烟叶风格差异的关键香气成分。(3)毕节各产地烟叶的2-苯乙醇和2,5-二甲基-4-羟基-3(2H)-呋喃酮的相对含量均较高，这可能是毕节烟区烟叶的蜂蜜味和甜味较为突出的主要原因。
Abstract：To investigate the differences in sensory quality and the key aroma components in tobacco leaves from different tobacco planting areas in Bijie, 42 samples of C3F flue-cured tobacco leaves were collected from 8 tobacco planting counties(districts or cities) in Bijie. The sensory quality of the samples was evaluated and the aroma components in the samples were analyzed by headspace solid-phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS). The results showed that:1) There were clear differences in sensory quality of tobacco leaves from the different areas, and the quality of tobacco leaves from the central and western areas was generally higher than the eastern area.2) A total of 318 volatile components were identified, in which 13 aroma components with VIP(Variable projection value) > 1 and rOAV(Relative odor activity value) > 1 were screened by using OPLS-DA model analysis and r OAV analysis. Furthermore, the key aroma components contributing to the leaf style difference from the different areas in Bijie were identified through the correlation analysis of the sensory quality indexes, they included β-damascenone, geranyl acetone, cis-3-hexenyl valerate,2,5-dimethyl-4-hydroxy-3(2H)-furanone, 2-phenylethanol, and phenylacetaldehyde. 3) The relative contents of 2-phenylethanol and 2,5-dimethyl-4-hydroxy-3(2H)-furanone in tobacco leaves from the different areas in Bijie were higher, which contributed to the honey and sweetness aroma notes which were prominent in Bijie tobacco leaves.
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基本信息:
DOI：10.16135/j.issn1002-0861.2024.0211
中图分类号:TS411;O657.63
引用信息:
[1]方嘉炜,严锦申,钟帅等.毕节烟区不同产地烟叶的感官质量特征及关键香气成分[J].烟草科技,2024,57(08):21-31.DOI:10.16135/j.issn1002-0861.2024.0211.
基金信息:
广东中烟工业有限责任公司科技项目“毕节烟叶质量特征分析及适配技术研究与应用”（2022440000340039）