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2025 05 v.58 26-33
非生物逆境胁迫对苗期烟草NtGGPPS家族基因表达及西柏三烯二醇含量的影响
基金项目(Foundation): 国家重点研发青年科学家项目“盐碱地适生多样性种植协同改土抗逆机制与调控技术研究”(2023YFD1901900); 中国农业科学院科技创新工程项目“基于代谢流调控及C-O键构建的萜烯成分生物合成”(ASTIP-TRIC-ZD03);中国农业科学院科技创新工程项目(ASTIP-TRIC06);中国农业科学院科技创新工程-中国农业科学院重大科技任务项目“盐碱地分区改良和综合利用技术集成示范”(CAAS-ZDRW202407)
邮箱(Email): xuzongchang@caas.cn;
DOI: 10.16135/j.issn1002-0861.2024.0898
中文作者单位:

中国农业科学院烟草研究所海洋农业研究中心;青岛市滨海盐碱地资源挖掘与生物育种重点实验室;国家盐碱地综合利用技术创新中心;山东中烟工业有限责任公司技术中心;

摘要(Abstract):

为揭示非生物逆境胁迫对苗期烟草西柏三烯二醇积累的影响机制,设置低温、高温、干旱和盐胁迫4种非生物逆境胁迫处理,并分析了烟苗萜烯类物质合成途径关键酶GGPPS家族成员的表达规律以及西柏三烯二醇含量(质量分数)的变化。结果表明,低温和高温胁迫均显著抑制NtGGPPS家族基因的表达,且表达量在42℃条件下最低;干旱胁迫也抑制了NtGGPPS家族基因的表达;盐胁迫显著抑制NtGGPPS1、NtGGPPS2和NtGGPPS4基因的表达;高温、干旱及盐胁迫能够诱导NtCYP71D16基因的表达。烟苗的西柏三烯二醇含量在高温、干旱和盐胁迫条件下显著提高。因此,多种非生物逆境胁迫条件可改变NtGGPPS家族成员以及西柏三烯二醇合成酶基因NtCYP71D16的表达模式,烟苗中西柏三烯二醇积累量也发生了变化。

关键词(KeyWords): 烟草;NtGGPPS基因;盐碱地;非生物逆境胁迫;高温;低温;干旱;盐
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基本信息:

DOI:10.16135/j.issn1002-0861.2024.0898

中图分类号:S572

引用信息:

[1]孟晨,鲁雪莉,朱海等.非生物逆境胁迫对苗期烟草NtGGPPS家族基因表达及西柏三烯二醇含量的影响[J].烟草科技,2025,58(05):26-33.DOI:10.16135/j.issn1002-0861.2024.0898.

基金信息:

国家重点研发青年科学家项目“盐碱地适生多样性种植协同改土抗逆机制与调控技术研究”(2023YFD1901900); 中国农业科学院科技创新工程项目“基于代谢流调控及C-O键构建的萜烯成分生物合成”(ASTIP-TRIC-ZD03);中国农业科学院科技创新工程项目(ASTIP-TRIC06);中国农业科学院科技创新工程-中国农业科学院重大科技任务项目“盐碱地分区改良和综合利用技术集成示范”(CAAS-ZDRW202407)

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