nav emailalert searchbtn searchbox tablepage yinyongbenwen piczone journalimg journalInfo journalinfonormal searchdiv searchzone qikanlogo popupnotification paper paperNew
2019, 02, v.52;No.384 12-18+61
秸秆还田和腐熟有机肥对植烟土壤养分、酶活性及微生物多样性的影响
基金项目(Foundation): 中国烟草总公司科技重点项目“基于宏基因学的植烟土壤健康评价研究与应用”(110201402016);中国烟草总公司科技重点项目“湖北烟区土壤保育及修复技术研究与应用”(110201502014)
邮箱(Email): kjzx737@126.com;
DOI: 10.16135/j.issn1002-0861.2018.0255
摘要:

为探讨不同有机物料对植烟土壤的改良效应,通过2015—2017年的田间定位小区试验,研究了水稻秸秆、玉米秸秆、烟草秸秆还田和腐熟有机肥对植烟土壤养分、酶活性、微生物数量和功能性菌群的影响。结果表明:秸秆还田和腐熟有机肥可提高土壤速效钾、有机碳、有效锌、有效镁和有效硼含量(质量分数);土壤蔗糖酶、脲酶和磷酸酶活性呈增加趋势,较对照分别提高55.0%、25.1%和129.5%,以腐熟有机肥处理的效果最好。秸秆还田和腐熟有机肥处理的土壤微生物OTUs总量为8 038~8 643,较对照提高7.4%~15.5%。施用腐熟有机肥还提高了土壤微生物群落多样性和丰富度,降低固氮菌微生物的相对丰度;而秸秆还田降低了土壤硝化功能菌和反硝化功能菌微生物的相对丰度,提高固氮菌微生物的相对丰度和土壤微生物固氮水平。秸秆还田和腐熟有机肥可降低土壤中烟草致病菌的相对丰度,腐熟有机肥处理较对照降低37.9%,且降低烟草感病风险的效果优于秸秆还田。综合分析,腐熟有机肥还田比秸秆还田对烟叶生产更为有利。

Abstract:

To investigate the effects of different organic materials on soil amelioration, a field experiment was conducted from 2015 to 2017. The effects of rice straw returning, maize stalk returning, tobacco stalk returning and decomposed organic manure on the nutrient contents, enzyme activities, microbial quantity and functional microbes in tobacco-planting soils were studied. The results showed that straw returning and decomposed organic manure increased the contents of organic carbon, available potassium, zinc, magnesium and boron.Comparing with the control, the activities of invertase, urease and phosphatase were increased by 55.0%, 25.1%and 129.5%, respectively. Especially, decomposed organic manure had an excellent effect. Under the treatments of straw returning and decomposed organic manure, the total amount of OTUs of soil microbes were 8 038-8 643 and increased by 7.4%-15.5% comparing with the control. Decomposed organic manure promoted the community diversity and abundance of microbes and decreased the relative abundance of nitrogen-fixing microbes. Straw returning decreased the relative abundances of nitrification and denitrification microbes and improved the relative abundance of nitrogen-fixing microbes and their nitrogen-fixing ability. Straw returning and decomposed organic manure reduced the relative abundance of pathogenic bacteria in tobacco-planting soils, decomposed organic manure decreased by 37.9% comparing with the control, and its susceptible risk reducing effect was better than straw returning. In conclusion, the effects of decomposed organic manure were morebeneficialtotobacco

参考文献

[1]谭周进,李倩,李建国,等.稻草还田量对晚稻土微生物数量及活度的动态影响[J].农业环境科学学报,2006,25(3):670-673.TAN Zhoujin,LI Qian,LI Jianguo,et al.Effect of returning quantity of rice-straw to soil on quantities and activity of microbial in paddy soil[J].Journal of Agro-Environment Science,2006,25(3):670-673.

[2]赵其国,钱海燕.低碳经济与农业发展思考[J].生态环境学报,2009,18(5):1609-1614.ZHAO Qiguo,QIAN Haiyan.Low carbon economy and thinking of agricultural development[J].Ecology and Environmental Sciences,2009,18(5):1609-1614.

[3]李军营,邓小鹏,杨坤,等.施用有机肥对植烟土壤理化性质的影响[J].中国土壤与肥料,2012(3):12-16,34.LI Junying,DENG Xiaopeng,YANG Kun,et al.Effect of organic fertilizer on soil physicochemical property in tobacco field[J].Soil and Fertilizer Sciences in China,2012(3):12-16,34.

[4]汪军,王德建,张刚.秸秆还田下氮肥用量对稻田养分淋洗的影响[J].中国生态农业学报,2010,18(2):316-321.WANG Jun,WANG Dejian,ZHANG Gang.Effect of incorporated straw-nitrogen fertilizer on nutrient leaching in paddy soils[J].Chinese Journal of Eco-Agriculture,2010,18(2):316-321.

[5]Harris J A,Birch P.Soil microbial activity in opencast coal mine restorations[J].Soil Use and Management,1989,5(4):155-160.

[6]萨如拉,高聚林,于晓芳,等.玉米秸秆深翻还田对土壤有益微生物和土壤酶活性的影响[J].干旱区资源与环境,2014,28(7):138-143.SA Rula,GAO Julin,YU Xiaofang,et al.Effect of,t t t straw-deep incorporation on soil beneficial microorganism and soil enzyme activities[J].Journal of Arid Land Resources and Environment,2014,28(7):138-143.

[7]龙健,黄昌勇,滕应,等.红壤矿区复垦土壤的微生物生态特征及其稳定性恢复研究Ⅱ.对土壤微生物生态特征和群落结构的影响[J].应用生态学报,2004,15(2):237-240.LONG Jian,HUANG Changyong,TENG Ying,et al.Microbial eco-characterization and its reclaimed wasteland in red soil area of ChinaⅡ.Effects on soil microbial characteristics and community structure[J].Chinese Journal of Applied Ecology,2004,15(2):237-240.

[8]Alexander K,Bryans T.Evaluation of the sterility test for detection of microbial contaminants of allografts[J].Cell and Tissue Banking,2006,7(1):23-28.

[9]刘海,杜如万,赵建,等.施肥对烤烟根际土壤酶活性及细菌群落结构的影响[J].烟草科技,2016,49(10):1-8.LIU Hai,DU Ruwan,ZHAO Jian,et al.Effects of fertilization on enzyme activities and bacterial community structures in rhizosphere soil of flue-cured tobacco[J].Tobacco Science&Technology,2016,49(10):1-8.

[10]房焕,李奕,周虎,等.稻麦轮作区秸秆还田对水稻土结构的影响[J].农业机械学报,2018,49(4):297-302.FANG Huan,LI Yi,ZHOU Hu,et al.Effects of straw incorporation on paddy soil structure in rice-wheat rotation system[J].Transactions of the Chinese Society for Agricultural Machinery,2018,49(4):297-302.

[11]张聪,慕平,尚建明.长期持续秸秆还田对土壤理化特性、酶活性和产量性状的影响[J].水土保持研究,2018,25(1):92-98.ZHANG Cong,MU Ping,SHANG Jianming.Effects of continuous returning corn straw on soil chemical properties,enzyme activities and yield trait[J].Research of Soil and Water Conservation,2018,25(1):92-98.

[12]闫宁,郭东锋,姚忠达,等.烟草秸秆还田对土壤细菌多样性的影响[J].江西农业学报,2016,28(5):40-45.YAN Ning,GUO Dongfeng,YAO Zhongda,et al.Effects of tobacco stalk returning on soil bacterial diversity[J].Acta Agriculturae Jiangxi,2016,28(5):40-45.

[13]鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,2000.BAO Shidan.Soil and agricultural chemistry analysis[M].3rd ed.Beijing:China Agriculture Press,2000.

[14]李振高,骆永明,腾应.土壤与环境微生物研究法[M].北京:科学出版社,2008.LI Zhengao,LUO Yongming,TENG Ying.Soil and environmental microbiological research method[M].Beijing:Science Press,2008.

[15]贺纪正,袁超磊,沈菊培,等.土壤宏基因组学研究方法与进展[J].土壤学报,2012,49(1):155-164.HE Jizheng,YUAN Chaolei,SHEN Jupei,et al.Methods for and progress in research on soil metagenomics[J].Acta Pedologica Sinica,2012,49(1):155-164.

[16]Edgar R C.Search and clustering orders of magnitude faster than BLAST[J].Bioinformatics,2010,26(19):2460-2461.

[17]GB/T 23222-2008烟草病虫害分级及调查方法[S].GB/T 23222-2008 Grade and investigation method of tobacco diseases and insect pests[S].

[18]Chao A N,Bunge J.Estimating the number of species in a stochastic abundance model[J].Biometrics,2002,58(3):531-539.

[19]兰雪,戴全厚,喻理飞,等.喀斯特退化森林不同恢复阶段土壤酶活性研究[J].农业现代化研究,2009,30(5):620-624.LAN Xue,DAI Quanhou,YU Lifei,et al.Soil enzyme activity of different restoration stages in Karst degenerative forest[J].Research of Agricultural Modernization,2009,30(5):620-624.

[20]潘剑玲,代万安,尚占环,等.秸秆还田对土壤有机质和氮素有效性影响及机制研究进展[J].中国生态农业学报,2013,21(5):526-535.PAN Jianling,DAI Wanan,SHANG Zhanhuan,et al.Review of research progress on the influence and mechanism of field straw residue incorporation on soil organic matter and nitrogen availability[J].Chinese Journal of Eco-Agriculture,2013,21(5):526-535.

[21]田雁飞,马友华,胡园园,等.秸秆肥料化生产的现状、问题及发展前景[J].中国农学通报,2010,26(16):158-163.TIAN Yanfei,MA Youhua,HU Yuanyuan,et al.The present state,problems and developing perspectives on fertilizer production with straws[J].Chinese Agricultural Science Bulletin,2010,26(16):158-163.

[22]薄国栋,申国明,陈旭,等.秸秆还田对植烟土壤酶活性及细菌群落多样性的影响[J].中国烟草科学,2017,38(1):53-58.BO Guodong,SHEN Guoming,CHEN Xu,et al.Effect of straw returning on soil enzyme activities and diversity of bacterial communities in tobacco planting Fields[J].Chinese Tobacco Science,2017,38(1):53-58.

[23]杨敏芳,朱利群,韩新忠,等.耕作措施与秸秆还田对稻麦两熟制农田土壤养分、微生物生物量及酶活性的影响[J].水土保持学报,2013,27(2):272-275,281.YANG Minfang,ZHU Liqun,HAN Xinzhong,et al.Effects of tillage and crop residues incorporation on soil nutrient,microbial biomass and enzyme activity under rice-wheat rotation[J].Journal of Soil and Water Conservation,2013,27(2):272-275,281.

[24]董亮,田慎重,王学君,等.秸秆还田对土壤养分及土壤微生物数量的影响[J].中国农学通报,2017,33(11):77-80.DONG Liang,TIAN Shenzhong,WANG Xuejun,et al.Effect of straw turnover on soil nutrients and soil microbial population[J].Chinese Agricultural Science Bulletin,2017,33(11):77-80.

[25]路怡青,朱安宁,张佳宝,等.免耕和秸秆还田对土壤酶活性和微生物群落的影响[J].土壤通报,2014,45(1):85-90.LU Yiqing,ZHU Anning,ZHANG Jiabao,et al.Effects of no-tillage and returning straw to soil on soil enzymatic activities and microbial population[J].Chinese Journal of Soil Science,2014,45(1):85-90.

[26]袁铭章,刘树堂,陈延玲,等.16S rDNA扩增子测序揭示长期定位秸秆还田对土壤细菌群落的影响[J].华北农学报,2016,31(6):157-163.YUAN Mingzhang,LIU Shutang,CHEN Yanling,et al.Illumina amplicon sequencing of 16S rDNA tag reveals bacteria community development of long-term straw returning soil[J].Acta Agriculturae Boreali-Sinica,2016,31(6):157-163.

[27]范丙全,刘巧玲.保护性耕作与秸秆还田对土壤微生物及其溶磷特性的影响[J].中国生态农业学报,2005,13(3):130-132.FAN Bingquan,LIU Qiaoling.Effect of conservation tillage and straw application on the soil microorganism and P-dissolving characteristics[J].Chinese Journal of Eco-Agriculture,2005,13(3):130-132.

[28]杨志臣,吕贻忠,张凤荣,等.秸秆还田和腐熟有机肥对水稻土培肥效果对比分析[J].农业工程学报,2008,24(3):214-218.YANG Zhichen,LüYizhong,ZHANG Fengrong,et al.Comparative analysis of the effects of straw-returning and decomposed manure on paddy soil fertility betterment[J].Transactions of the CSAE,2008,24(3):214-218.

[29]邱强.作物病虫害诊断与防治彩色图谱[M].北京:中国农业科学技术出版社,2013.QIU Qiang.Color atlas of the diagnosis and control of crop pests and diseases[M].Beijing:China Agricultural Science and Technology Press,2013.

[30]Liu Y X,Shi J X,Feng Y G,et al.Tobacco bacterial wilt can be biologically controlled by the application of antagonistic strains in combination with organic fertilizer[J].Biology and Fertility of Soils,2013,49(4):447-464.

[31]黎妍妍,覃光炯,王林,等.清江流域烟区烟草青枯病发生的土壤营养因素分析[J].南方农业学报,2018,49(4):656-661.LI Yanyan,QIN Guangjiong,WANG Lin,et al.Soil nutrient elements affecting the occurrence of tobacco bacterial wilt in Qingjiang River Basin[J].Journal of Southern Agriculture,2018,49(4):656-661.

[32]Malhi S S,Nyborg M,Goddard T,et al.Long-term tillage,straw management,and nitrogen fertilization effects on organic matter and mineralizable carbon and nitrogen in a black chernozem soil[J].Communications in Soil Science and Plant Analysis,2012,43(20):2679-2690.

[33]Mazzola M,Strauss S L.Resilience of orchard replant soils to pathogen re-infestation in response to Brassicaceae seed meal amendment[J].Aspects of Applied Biology,2013,119:69-77.

[34]Workneh F,van Bruggen A H C.Microbial density,composition,and diversity in organically and conventionally managed rhizosphere soil in relation to suppression of corky root of tomatoes[J].Applied Soil Ecology,1994,1(3):219-230.

[35]施河丽,孙立广,谭军,等.生物有机肥对烟草青枯病的防效及对土壤细菌群落的影响[J].中国烟草科学,2018,39(2):54-62.SHI Heli,SUN Liguang,TAN Jun,et al.Control efficiency of bio-organic fertilizers on tobacco bacterial wilt and their effects on soil bacterial community[J].Chinese Tobacco Science,2018,39(2):54-62.

基本信息:

DOI:10.16135/j.issn1002-0861.2018.0255

中图分类号:S572;S141

引用信息:

[1]樊俊,谭军,王瑞,等.秸秆还田和腐熟有机肥对植烟土壤养分、酶活性及微生物多样性的影响[J].烟草科技,2019,52(02):12-18+61.DOI:10.16135/j.issn1002-0861.2018.0255.

基金信息:

中国烟草总公司科技重点项目“基于宏基因学的植烟土壤健康评价研究与应用”(110201402016);中国烟草总公司科技重点项目“湖北烟区土壤保育及修复技术研究与应用”(110201502014)

检 索 高级检索

引用

GB/T 7714-2015 格式引文
MLA格式引文
APA格式引文