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为阐明霍氏肠杆菌（Enterobacter hormaechei）提升发酵烟叶品质的代谢机制，利用非靶向代谢组学技术，系统分析了其固态发酵烟叶过程中驱动的代谢物转化规律和关键功能通路。以陕西宝鸡烟区C3F-AB 2022烟叶为材料，采用高效液相色谱-四极杆飞行时间质谱（HPLC-QTOF-MS）技术，结合偏最小二乘法判别分析（PLS-DA），筛选霍氏肠杆菌发酵烟叶0～72 h的差异代谢物，并通过KEGG通路揭示关键代谢途径。结果表明：(1)共鉴定到945个代谢物，筛选出151个显著差异代谢物，包括葡萄糖、氨基酸、有机酸和萜类化合物等。(2)差异代谢物富集于苯丙氨酸、酪氨酸和色氨酸生物合成，萜类和醌类生物合成等129条通路上。其中，5条通路（如萜类和醌类化合物生物合成、苯丙氨酸代谢、半乳糖代谢）与品质提升显著相关。非靶向代谢组学分析结果表明，霍氏肠杆菌的生长代谢通过富集于萜类合成、苯丙氨酸代谢等关键通路，驱动了差异代谢物的转化，提升了烟叶品质。
Abstract：To elucidate the metabolic mechanism of Enterobacter hormaechei in improving the quality of fermented tobacco leaves, non-targeted metabolomics technology was employed to systematically analyze the transformation patterns and key functional pathways of metabolites during the solid-state fermentation procedure of tobacco leaves. The high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(HPLC-QTOF-MS) technology was applied and partial least squares discriminant analysis(PLSDA) was conducted to screen differential metabolites in tobacco leaves(C3F-AB 2022) from Baoji tobaccoproducing area of Shaanxi Province fermented by Enterobacter hormaechei for 0-72 h. The key metabolic pathways were identified using KEGG pathway analysis. The results showed that: 1) A total of 945 metabolites were identified, 151 of which were significantly differential metabolites, including glucose, amino acids, organic acids, and terpenoids, etc. 2) The differential metabolites were enriched in 129 pathways, such as biosynthesis pathway of phenylalanine, tyrosine, and tryptophan and biosynthesis pathway of terpenoid and quinone. Among these, 5 pathways including biosynthesis of terpenoids and quinones, metabolism of phenylalanine and metabolism of galactose were significantly related with the leaf quality improvement. Non-targeted metabolomics analysis revealed that the growth and metabolism of Enterobacter hormaechei drive the transformation of differential metabolites by enriching in key pathways including terpenoids synthesis and phenylalanine metabolism, thereby improve the quality of tobacco leaves.
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基本信息:
DOI：10.16135/j.issn1002-0861.2025.0428
中图分类号:TS414
引用信息:
[1]王祯,杨红静,宋凯,等.基于非靶向代谢组学的霍氏肠杆菌固态发酵烟叶代谢物动态变化[J].烟草科技,2026,59(03):1-9.DOI:10.16135/j.issn1002-0861.2025.0428.
基金信息:
中国烟草总公司科技重点研发项目“加速烟叶醇化微生物资源发掘及其应用技术研究”（110202202006）; 甘肃省重点研发计划项目“烟草源微生物靶向增香提质技术研究”（22YF11GA292）; 中国烟草实业发展中心科技项目“基于微生物的烟叶清香型风格定向重构技术开发”（ZYSYQ-2022-7）
2026-01-15
2026-01-15
2026-01-15