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为确定香紫苏精油关键香气成分并构建风味轮廓图，采用气相色谱-质谱（GC-MS）、气相色谱-质谱-嗅闻联用（GC-MS-O）技术，结合ZB-5、ZB-WAX双柱定性分析香紫苏精油的香气成分，并对香气成分进行气相色谱-串联质谱-选择反应监测扫描（GC-MS-MS-SRM）定量分析，结合香气阈值，筛选出香紫苏精油的关键香气成分。结果表明：(1)两种色谱柱共嗅辨出27种香气活性化合物，其中，有8种化合物为两种色谱柱共同检出的成分。(2)香气活性化合物可系统划分为四大香韵类型（花香、果香、草本、木质），共同构成香紫苏精油的风味轮廓图，其中，花香和果香化合物的种类较多，风味稀释因子（FD）值较高；草本和木质香韵化合物的种类较少，FD值较低。(3)香气活力值（OAV）大于1的关键香气成分有17种，其中，主导花香、果香、草本和木质香韵的关键香气成分分别为芳樟醇、乙酸芳樟酯、月桂烯和β-石竹烯。
Abstract：To identify the key aroma components in Salvia sclarea L. essential oil and construct its flavor profile, gas chromatography-mass spectrometry(GC-MS) and gas chromatography-mass spectrometry-olfactometry(GC-MS-O)techniques were employed. These techniques combine dual-column(ZB-5 and ZB-WAX)systems to qualitatively analyze the aroma components in Salvia sclarea L. essential oil, followed by a quantitative analysis using gas chromatography-tandem mass spectrometry in selected reaction monitoring(GC-MS-MS-SRM)mode. The key aroma components were screened based on their odor thresholds. The results showed that: 1) A total of 27 aroma-active compounds were olfactorily discriminated using two chromatographic columns, eight of which were detected by both columns. 2)These aroma-active compounds could be systematically classified into four major aroma categories: floral,fruity, herbal and woody. These categories jointly formed the flavor profile of Salvia sclarea L. essential oil. Floral and fruity compounds were more abundant, exhibiting higher flavor dilution(FD) factors,whereas herbal and woody compounds were less abundant, exhibiting lower FD factors. 3) The odor activity values(OAVs)of the 17 key aroma compounds were all higher than 1. The dominant compounds representing the floral, fruity, herbal and woody aromas were linalool, linalyl acetate, myrcene andβ-caryophyllene, respectively.
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基本信息:
DOI：10.16135/j.issn1002-0861.2025.0092
中图分类号:TS264.3;O657.63
引用信息:
[1]陈伟华,刘子轩,贾晓慧,等.GC-MS-O-双柱定性分析香紫苏精油的关键香气成分[J].烟草科技,2025,58(09):13-22.DOI:10.16135/j.issn1002-0861.2025.0092.
基金信息:
河北中烟工业有限责任公司科技项目“荷花品牌功能性香精香料关键成分评价方法与应用研究”（HBZY2024A068）